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FreezeKit™ Cleave /ThawKit™ Cleave  

FreezeKit™ Cleave /ThawKit™ Cleave

FreezeKit™ Cleave  REF:10166

ThawKit™ Cleave   REF:10167

 

The new generation of slow freezing – optimised composition, convenient protocol and high survival rate
Outstanding results

 

Outstanding results
Two independent studies show that survival of pronuclear stage oocytes and cleavage stage embryos are significantly improved using the new FreezeKit Cleave and ThawKit Cleave.

Significantly higher blastocyst survival
GraphTwo clinics in Sweden made a pre-clinical, randomised comparison of FreezeKit Cleave and ThawKit Cleave with an existing commercial product for freezing of cleavage-stage embryos.

The primary endpoint was blastomere survival rate. A statistically significant difference in blastomere survival was seen between the two methods.

Significantly better survival rate
At Westmead Fertility Center Australia, clinical use of FreezeKit Cleave and ThawKit Cleave resulted in improved survival. A retrospective analysis on 1497 embryos from 377 patients showed significantly better survival rate for both 2PN- and cleavage-stage embryos.

Safe to use for previously frozen material
Survival of cleavage-stage embryos frozen with our older product, FREEZE-KIT 1, and thawed with the new ThawKit Cleave was 73%. This demonstrates the safety of the new procedure on previously frozen material.

New media composition
Based on our G-Series™ media with carefully selected amino acids for embryo metabolism.


MOPS bufferMOPS-buffered for more stable pH during handling and temperature shifts

HyaluronanHyaluronan and Human Serum Albumin are included for cryo protection.


Improved levels of cryoprotectants in freezing kit for better dehydration.


PrOH not present in the thawing media for reduced exposure time to penetrating cryoprotectant.

Time-saving and convenient
FreezeKit Cleave and ThawKit Cleave make the whole slow freezing procedure faster and more convenient. Fewer steps compared to current protocol save time, but also reduce operator variability and facilitates the process of training new staff. Most importantly is however that the fewer steps and shorter handling time lower the stress induced to the embryos.

Content in kit
1x10 mL - Thawing solution 1
1x10 mL - Thawing solution 2
1x10 mL - Equilibration solution

Note. This product is not available for sale in USA

 


 

Application field:
Purpose:For freezing of pronuclear oocytes and cleavage stage embryos/For thawing of frozen pronuclear oocytes and cleavage stage embryos
Description:MOPS buffered media
Application:For use in sequence after equilibration at room temperature and ambient atmosphere
Storage:Store dark at +2 to +8ºC

 

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